In determination of AFB 1 spiked in feed sample, TRFIA showed high accuracy and precision. Cross-reactivity with AFM 1, AFB 2, AFG 1 and AFG 2 was 31.26%, 37.6%, 127.46% and 35.74%, respectively, but zero with other analogues. Under optimal assay conditions, TRFIA was shown to be highly sensitive and specific in detection of AFB 1. Goat anti-mouse immunoglobulin G (IgG) was modified by Eu 3+-DATT, generating Eu 3+-labeled IgG. The 50% inhibition value (IC 50) was 0.81 ng mL −1, the limit of detection (LOD) was 0.10 ng mL −1 and detection range was 0.10–3.94 ng mL −1.
Monoclonal antibody (McAb) against AFB 1 (9B11-D7) was prepared through immunization and cell fusion and was identified as high affinity, specificity and sensibility by enzyme-linked immunosorbent assay (ELISA). A time-resolved fluoroimmunoassay (TRFIA) was established for quantitative detection of AFB 1 in feed using Eu 3+-labeled IgG as tracer. Aflatoxin B 1 (AFB 1) is a kind of toxic and carcinogenic mycotoxin.
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